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BIO103 Biology

Question:

Hypothesis

Does catfish water have more bacteria and colonies?

For an experiment to be conducted we sampled two areas with a cotton swab then transferred it to a growth medium suspended in agar. First we used sterile water to make the swab wet then sampled the aquarium right in the middle. We divided the petri dish into two sections using a marker. Section A, which is the catfish, and B, which is the minnows.

We gently transferred the bacteria to their respective places. Before the experiment we made sure not to open the petri dishes to avoid contamination with the air microbes. Immediately after collecting the samples we sealed the petri dish with tape and put it in a box for storage. The catfish aquarium looked murky and much dirtier than aquarium

1. Aquarium B was mainly the growth of Algae. The samples were incubated in a box at 37 degrees celsius warmer to get the growth for Two days then for the next five days it was placed in the fridge at 40 degrees celsius since the bacteria was growing way too fast.

2. After a week we observed the colonies. We divided the morpho species by their color, size, shape, growth, pattern, number and texture.

Catfish:

A. small round and tan

B. Smaller and brownish

Minnows:

C. flat, translucent , Halo

D. opaque

E. orange, brown, bigger circles

F. brown, not transparent, bleeding out

G. pink

F. irregular,rough edges, ( ice on a windshield).

Results show that Side A which is the catfish contains more bacterial colonies. The results support our hypothesis that the catfish

For an experiment to be conducted We sampled two areas with a cotton swab then transferred it to a growth medium suspended in agar. First, we used sterile water to make the swab wet then sampled the aquarium right in the middle. We divided the petri dish into two sections using a marker. Section A, which is the catfish, and B, which is the minnows. We gently transferred the bacteria to their respective places. Before the experiment, we made sure not to open the Petri dishes to avoid contamination with the air microbes. Immediately after collecting the samples we sealed the petri dish with tape and put it in a box for storage. The catfish aquarium looked murky and much dirtier than aquarium B.

Aquarium B was mainly the growth of Algae. The samples were incubated in a box at 37 degrees Celsius warmer to get the growth for Two days then for the next five days it was placed in the fridge at 40 degrees celsius since the bacteria was growing way too fast. After a week we observed the colonies. We divided the morphospecies by their color, size, shape, growth, pattern, number, and texture.

BIO103 Biology

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