Your company is growing plants that produce very valuable chemical X in roots. Genes responsible for the biosynthesis of this metabolite are Gene XA, GeneXB
The post Gene technology first appeared on COMPLIANT PAPERS.
Your company is growing plants that produce very valuable chemical X in roots. Genes responsible for the biosynthesis of this metabolite are Gene XA, GeneXB and GeneXC. These genes express only in roots because of the root-specific transcription factor, TFX, which regulates them.
These plants cannot be used for the commercial production of X because:
Collecting the roots will kill plants, so you need to re-grow them often;
Roots are small in sizes (root’s biomass is not big)
As a result, it is not easy to collect enough biomass from roots every year for the commercial production of X.
To increase the production of the chemical X your company decided to re-direct the production of chemical X from roots to leaves of the same plants. The rationales behind this decision are:
These plants have big leaves,
To collect leaves, you do not need to kill plants and re-grow them,
Leaves and roots have the same genome, so all genes involved in the production of X exist in leaves.
You are the lucky postdoc who has to run this project. You need to designe a strategy to conduct this project. For commercial production of the generated transgenic plants under field conditions, they have to meet modern (advanced) genetic engineering conditions.
You need to:
1. Design a transgene and explain its every component (you can design more than one transgene): 20 marks
2. Design the vector that contains your transgene: 20 marks
3. Design a strategy for the generation of the transgenic plant: 20 marks
4. Design a strategy to confirm your generated plant: 10 marks
5. Expression of genes X in leaves could change the expression profiles of other genes. How can you assess the global molecular changes in leaf cells? : 20 marks
6. Which adverse effects do you expect in your experiment: 10 marks?
The post Gene technology first appeared on COMPLIANT PAPERS.
