Identification of a Pathogenic Bacterium using PCR & DNA Sequencing
Week 4 Lab Questions –
1. What does the term, “Pathogenic” mean?
2. What are “Proteolytic Enzymes”?
3. What does it mean to “denature” an enzyme?
4. What is PCR?
5. What did the “PCR Master Mix” contain?
6. What is the technical term for a “PCR Machine”?
7. What three steps are carried out during each cycle of a PCR?
8. What color identified the microconcentrator column?
9. What was the reason for running the second PCR run during sequencing prep?
10. During sequencing, the smallest fragments of DNA will run through the gel ___________.
(fastest/slowest)
11. What does the acronym “BLAST” stand for?
12. What species of bacterium is this patient infected with? ________________
(this should be the organism whose DNA alignment most closely matches the one you submitted in your BLAST search). (to confirm your answer, complete the HHMI Virtual Lab)
13. How might this patient have contracted this specific bacterium?
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