BIO230 Enzyme Assignment (20 pts)
This assignment makes use of the following website to run some simulations:
https://sites.google.com/site/biologydarkow/enzymes/testing-unknown-enzymes
Part 1. Use the simulation to match enzyme with substrate (3 pts)
The default view for this simulation is the Enzyme Catalysis (line) view, which tracks the dynamics of the enzyme reaction over a 20 minute simulated time period. You can also view the Enzyme Catalysis (bar) tab, that will only give a bar graph of the Product Concentration generated for each enzyme.
There are 4 enzymes in this simulation, which are present at a constant concentration.
Using the sliders on the left side of the screen, however, you have the option of changing the concentrations of each substrate, the temperature of the reaction, and the pH of the reaction.
You can view the enzyme kinetics “live” by clicking the “Live” button. You can then see the kinetic change as you move the different left sliders.
You can also view a single “Run” of the reaction with all four enzymes. “Runs” are saved and you can view all the Runs for each enzyme by clicking on the Tab for each enzyme at the top.
One at a time, move the sliders to match each enzyme with its substrate. You may need to adjust the temperature or pH, as not all enzymes may be active under identical conditions.
Enzyme 1 _______________
Enzyme 2 _______________
Enzyme 3 _______________
Enzyme 4 _______________
Part 2. Collect data to determine the optimum temperature and pH conditions for each enzyme.
In order to match enzyme to substrate, you likely had to adjust temperature and pH. For each enzyme, write a temperature and pH combination for which you found the enzyme to be able to generate product.
Enzyme 1 _______________
Enzyme 2 _______________
Enzyme 3 _______________
Enzyme 4 _______________
Product Concentration Vs pH
You will complete Table 1 below (2 pts):
Table 1: Product Concentration Vs pH
enzyme 1
enzyme 2
enzyme 3
enzyme 4
substrate and concentration
temperature used:
pH:
Use the temperature listed in the previous section above and choose a concentration to test for each substrate.
Choose 10 different pH values that you will use to test all of the enzymes. Your range should be broad enough to ensure each enzyme will be active in at least one pH.
At each pH value, record the Product Concentration (what are the units?) for each enzyme. Enter the results in the table above.
Graph your pH Results. (2 pts)
Using either Excel or Google sheets, make a graph of the results from Table 1. Your graph should have
a Title, similar to that of Table 1.
pH on the X axis
Product Concentration (with units) on the Y axis
Each enzyme represented as a line, with each line clearly visible and labeled
Analysis and Conclusion (Paragraph Format, 4 pts).
Based on your results, describe how the pH affects the activity of each enzyme.
For each enzyme, describe whether the enzyme is active at a broad range or a narrow range of pH values and what would you estimate is the “optimum” pH for each enzyme.
Based on the pH range, could this enzyme be found in an environment in the human body?
Use outside sources and the optimum pH value to search for possible areas in cells or organs that might match the optimum pH for each enzyme. (Note, the simulated enzymes may match enzymes found in non-human organisms)
Include a citation for the sources that you use.
Product Concentration Vs Temperature
You will complete Table 2 below (2 pts):
Table 2: Product Concentration Vs Temperature
enzyme 1
enzyme 2
enzyme 3
enzyme 4
substrate and concentration
pH used:
temp:
Use the optimum pH identified in the previous section above and keep the same substrate concentration.
Choose 10 different temperature values that you will use to test all of the enzymes. Your range should be broad enough to ensure each enzyme will be active in at least one temperature.
At each temperature value, record the Product Concentration (what are the units?) for each enzyme. Enter the results in the table above.
Graph your Temperature Results (2 pts).
Using either Excel or Google sheets, make a graph of the results from Table 2. Your graph should have
a Title, similar to that of Table 2.
Temperature on the X axis
Product Concentration (with units) on the Y axis
Each enzyme represented as a line, with each line clearly visible and labeled
Analysis and Conclusion (Paragraph Format, 5 pts).
Based on your results, describe how the temperature affects the activity of each enzyme.
For each enzyme, describe whether the enzyme is active at a broad range or a narrow range of temperature values and what would you estimate is the “optimum” temperature for each enzyme.
For temperatures at which the enzyme is not active, what is the reason? Is it likely dependent upon the variation of molecular collisions with temperature or the shape of the enzyme (denaturing)?
Now that you have optimized values for both pH and temperature, return to the question: Could this enzyme be found in an environment in the human body?
Use outside sources and the optimum temperature value to search for possible areas in cells or organs that might match the optimum temperature for each enzyme. (Note, the simulated enzymes may match enzymes found in non-human organisms)
Include a citation for the sources that you use.
Based on information you can find, suggest a hypothesis about the identify of enzyme and or organism.
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