1. (10 points) From a kinetics experiment, keat was determined to be 195sec”. For the kinetic assay, 0.3mL of a 0.35mg/mL solution of enzyme was used, and the enzyme has a molecular weight of 75,000 g/mole. Assume a reaction volume of 3mL. Calculate Vmax (UM’min’) for the enzyme and catalytic efficiency in M’sec ) for the enzyme. The Kn for the enzyme was determined to be 2.5 x 10″-M.