Microscopy
Review parts and their functions of the binocular compound light microscope
A compound light microscope has ( 1 / 2 / 3 ) lenses to magnify an object.
Record magnification power for your microscope:
ocular or eyepiece _____
scanning objective _________
low power objective _______
high power objective _____
oil-immersion objective ________
Calculate total magnification when using
Scanning objective : _________ X ________ = _________
Low power objective : _________ X ________ = _________
High power objective : _________ X ________ = _________
Oil immersion objective : _________ X ________ = _________
As magnification increases resolution ( increases / decreases / stays the same) up to a limit when the image becomes fuzzy. Which objective lens will give you the greatest resolving power?________________
As magnification increases, the field of view ( increases / decreases / stays same)
Letter “e” slide
Draw Appearance of letter “e” on slide to unaided eye: _______
When viewed through microscope, how does this e appear? ______________________
When the slide moves right, in which direction does the specimen move? _____________
To move a specimen to the upper left hand field of view which way must one move the slide?_____________
Illumination slide
As you increase the magnification of the specimen, how does the amount of light transmitted change? ___________ What, if any, change do you need to make to have a good image in the field of view as you increase magnification? _____________________________________________
Focus and Depth Colored thread slide
As magnification increases then the depth of field (increases / decreases / stays the same)
What color thread is on top of the others?________________
If 2 paramecia equal 1 mm, then each is 0.5 mm. 1 mm = 1000 micrometers. How many micrometers is 1 paramecium long? _________________________
Distance and Diameter Stage micrometer slide
Use low power objective. Record # of millimeters (to tenths) that you see along the field of view: Convert the # that you attained to micrometers (1 millimeter = 1,000 micrometers) ______________ = LPD
LPD = diameter of field of view for low power objective HPD = diameter of field of view for high power objective
LPM = low power magnification HPM = high power objective
HPD = LPD/ 1 (x) LPM/HPM = ____________ x __________ = ___________ micrometers ( microns)
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